Purification of Tubulin from Porcine Brain and its Fluorescence Dye Modification.
Methods in molecular biology (Clifton, N.J.)(2022)
Abstract
Filamentous microtubules, polymers of the heterodimeric protein tubulins play one of the major roles in the emergent nano-biotechnological devices. To develop the feature of those devices, it is important to understand the function of microtubule in in vitro, hence, the availability of purified αβ-tubulin is required. Additionally, fluorescently labeled tubulin has become a powerful approach for extensively studying the dynamics of these components. In this chapter, the process of purifying the heterodimeric αβ-tubulin from porcine brain will be described, as well as the process of labeling of the purified tubulin with fluorescence dye.
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Key words
Fluorescent dye,Labeling,Porcine brain,Purification,Tubulin
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