Exploring the mechanism of Qinbaiqingfei-concentrate pills in the treatment of Mycoplasma pneumoniae pneumonia from the perspective of intestinal microbiota and mucosal immunity

The view of traditional Chinese medicine for treating pneumonia is similar to the current “gut-lung axis” theory. We use this theory to study the mechanism for Qinbaiqingfei Concentrate Pills to treat mycoplasma pneumonia. First, establish a mycoplasma pneumonia model to detect mycoplasma content in lung tissue, inflammatory factors IL-4, IL-10, TNF-α, INF-γ and NFkB protein expression in lung tissue to understand whether Qinbai has the ability to treat diseases. Next, 16SrDNA technology is used to detect the intestinal flora. GC-MS is used to detect the content of short-chain fatty acids in feces to study the ability of drugs to regulate the intestinal microecology. Finally, it was decided to detect the indicators of mucosal immunity, detect HDAC, LPS, SIgA, etc., to facilitate the correlation of the overall experimental indicators. Through a series of experiments, it is confirmed that the drug can cure the disease by restoring the intestinal microecology and inhibiting the proliferation of mycoplasma.Image 1