Development and field evaluation of a nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) analysis to identify A. marginale-infected and A. centrale-vaccinated cattle

María Evangelina Primo,Julio Bellezze,Nicolas Morel,Matilde Mazzucco Panizza,Beatriz Susana Valentini,Susana Marta Torioni,Carolina Soledad Thompson

Ticks and Tick-borne Diseases（2022）

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摘要

•nPCR-RFLP detected the same number of DNA copies and PE as species-specific nPCR.•Strength of agreement between nPCR-RFLP and nPCR-Ac was “very good”.•Strength of agreement between nPCR-RFLP and nPCR-Am was “moderate”.•nPCR-Am failed to detect some genotypes present in Argentina.

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关键词

Anaplasma marginale,Anaplasma centrale,nPCR-RFLP

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