Complete genome sequence of the industrial l -lysine production strain [ Brevibacterium ] flavum CCM 251

[ Brevibacterium ] flavum CCM 251 is used to produce l -lysine in the Biotika biotech factory in Slovenská Ľupča, Slovakia. [ B. ] flavum CCM 251 is the exclusive host of the lytic corynephage BFK20, which has been extensively studied. We have sequenced the complete genome of [ B. ] flavum CCM 251 using a combination of the Illumina MiSeq platform and MinION technology. The genome comprised a circular chromosome of 3362281 bp with 54.13% G + C content. In this study, we present an analysis of this genome. We identified six prophage-related sequence regions; three of these regions were classified as incomplete and the three others as questionable. The prophages were classified as Caudovirales , family Siphoviridae . In silico analyses of the genome identified two complete aspartate biosynthetic pathways involved in l -lysine production, a direct variant and a succinylase variant. The complete genome sequence of [ B .] flavum CCM 251 (GenBank, CP079240) was compared with the genomes of other Corynebacterial strains. We confirmed these related strains have high similarity: 98.12% homology to C. glutamicum ATCC 13032 (EMBL BA00036) and 99.98% homology to [ B .] flavum ATCC 15168 and C. glutamicum BE.