Pseudomonas tumuqii sp. nov., isolated from greenhouse soil

A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, named LAMW06 T , was isolated from greenhouse soil in Beijing, China. In the 16S rRNA gene sequence comparison, strain LAMW06 T had the highest similarity with Pseudomonas cuatrocienegasensis 1N T . Phylogenetic analysis based on the 16S rRNA and three housekeeping gene sequences ( gyrB , rpoB and rpoD ) indicated that strain represented a member of the genus Pseudomonas . The genome sequence size of the isolate was 5.5 Mb, with a DNA G + C content of 63.5 mol%. The average nucleotide identity and DNA–DNA hybridization values between strain LAMW06 T and closely related members of Pseudomonas borbori R-20821 T , Pseudomonas taeanensis MS-3 T and P. cuatrocienegasensis 1N T were 90.9%, 82.4%, 81.5% and 43.0%, 25.9%, 24.6% respectively. The major fatty acids contained summed feature 3 (C 16:1 ω 6c and/or C 16:1 ω 7c), C 18:1 ω 7c and C 16:0 . The primary respiratory quinone was ubiquinone-9. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, six aminophospholipids, six phospholipids, one aminolipid and one glycolipid. According to the genotypic, phylogenetic and chemotaxonomic data, strain LAMW06 T represents a novel species within the genus Pseudomonas , for which the name Pseudomonas tumuqii sp. nov. is proposed. The type strain is LAMW06 T (= GDMCC 1.2003 T = KCTC 72829 T ).