Description and genome analysis of Luteimonas viscosa sp. nov., a novel bacterium isolated from soil of a sunflower field

Strain XBU10 T was isolated from a soil sample of a sunflower plot in Inner Mongolia, China. The isolate was a Gram-stain-negative, aerobic, non-motile, rod-shaped bacterium, and its colonies were bright yellow in colour. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XBU10 T belonged to the genus Luteimonas of the family Lysobacteraceae and was most closely related to Luteimonas panaciterrae Gsoil 068 T (97.8%), Luteimonas marina FR1330 T (97.6%), Luteimonas aquatica RIB1-20 T (97.4%) and Luteimonas huabeiensis HB2 T (97.2%). Growth occurred at 4–40 °C (optimum, 28–30 °C), with 0–5.0% (w/v) NaCl (optimum, 0.5%) and at pH 6.0–10.0 (optimum, pH 7.0 − 8.0). The chemotaxonomic characteristics of strain XBU10 T , which had Q-8 as its predominant quinone and iso-C 17:1 ω 9 c , iso-C 15:0 , iso-C 17:0 and iso-C 16:0 as its major fatty acids, were consistent with classification in the genus Luteimonas . The polar lipid profile of strain XBU10 T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, two unidentified aminophospholipids and three unidentified polar lipids. The genome of strain XBU10 T was 4.17 Mbp with a G + C content of 69.9%. Its genome sequence showed genes encoding alkaline phosphatase and catalase. Protein-coding genes related to carbohydrate-active enzymes were also observed. Average nucleotide identity (ANI) values between XBU10 T and other species of the genus Luteimonas were found to be low (ANIm < 88.0%, ANIb < 85.0% and OrthoANIu < 85.0%). Furthermore, digital DNA-DNA hybridization (dDDH) and average amino acid identity (AAI) values between strain XBU10 T and the closely related species ranged from 20.3 to 28.9% and from 64.2 to 82.3%, respectively. Based on the results of our phylogenetic, phenotypic, genotypic and chemotaxonomic analyses, it is concluded that strain XBU10 T represents a novel species within the genus Luteimonas , for which the name Luteimonas viscosa sp. nov. is proposed. The type strain is XBU10 T (= CGMCC 1.12158 T = KCTC 23878 T ).