Microfluidic system with light intensity filters facilitating the application of photodynamic therapy for high-throughput drug screening

•Microfluidic device performs single parallel test for different light irradiations.•Eight different intensities are generated from a single light-emitting diode.•Eight different color dye concentrations function as light intensity filters.•Optimum light intensities and photosensitizer doses were determined for cell lines.•High-throughput microfluidic system analyzes effects of light intensity during PDT.