Deep learning colorful ptychographic iterative engine lens-less diffraction microscopy

•An improved PIE based on computational deep learning color-transferring method is proposed for achieving colorful large FOV lens-less microscopy imaging.•The image data are three times less than those images under multiple illuminations for colorful PIE microscopy.•The color reconstructing time is very short, i.e., ∼7 ms.•The reconstructed color is very close to the ‘true data’, where is 2.7105.