Fungal digestive enzymes promote macronutrient hydrolysis in the INFOGEST static in vitro simulation of digestion

•An adapted method to study digestive enzymes in vitro has been developed.•Fungal enzymes are active under acidic conditions that model human stomach physiology.•Fungal enzymes improve dietary protein, fat, and carbohydrate digestion in vitro.•An adapted in vitro method has been established to study digestive decline with aging.•An adapted in vitro method has been established to study the effects of proton pump inhibitors on digestion.