Escherichia coli tRNA 2-selenouridine synthase SelU selects its prenyl substrate to accomplish its enzymatic function

Patrycja Szczupak,Ewa Radzikowska-Cieciura,Katarzyna Kulik,Rafał Madaj,Małgorzata Sierant,Agnieszka Krakowiak,Barbara Nawrot

Bioorganic Chemistry（2022）

Cited 4|Views14

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Abstract

•2-Selenouridine-tRNA synthase (SelU) is a two-faced enzyme that catalyzes S2U → Se2U.•SelU selects its specific prenyl substrate to produce geranylated S2U-tRNA.•Geranyl pyrophosphate (gePP) binds to SelU in the micromolar range.•The 7-aa fragment of the P-loop of SelU is a domain for recognition of gePP.•Methyl and various S-prenyl tRNAs serve as SelU substrates for selenation in vitro.

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Key words

Wobble nucleoside,Modified nucleoside,2-thiouridine,S2U,2-selenouridine,Se2U,tRNA 2‐selenouridine synthase,SelU,Geranyl,Prenyl

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