Time Course Metabolite Profiling of Fusarium Head Blight-InfectedHard Red Spring Wheat Using Ultra-High-Performance LiquidChromatography Coupled with Quadrupole Time of Flight/MS br

Wheat is an important food crop, yet its value is reduced by fungal infections (ex.Fusarium graminearum). Metaboliteprofiling is a useful tool for explaining resistance mechanisms. By analyzing near-isogenic lines (NILs) with contrastingFhb1allelesand three wheat varieties, a time course resulting in 61 relevant metabolites was studied. The presence of one metabolite as resistantrelated constitutive late in the time course was detected. Results confirm the presence of hydroxycinnamic acid amides conjugatedwith polyamine derivatives (hydroxycinnamic acid amides, HCAAs), which have been shown to induce thickening of cell walls.These compounds are shared by resistant and susceptible genotypes with no difference in intensities but vary in time as early- or late-occurring, suggesting that for the NIL studied here, HCAAs were a normal part of the host reaction. Overall, metabolites synthesizedas a result of infection were observed regardless of susceptibility but occurred at different times after infection