Cell-Based Assays to Identify Modulators of Nrf2/ARE Pathway.
Methods in molecular biology (Clifton, N.J.)（2022）
Abstract
The nuclear factor erythroid 2-related factor (Nrf2) and antioxidant response element (ARE) signaling pathway play an important role in the amelioration of cellular oxidative stress. Thus, assays that detect this pathway can be useful for identifying chemicals that induce or inhibit oxidative stress signaling. This chapter is to describe two cell-based Nrf2/ARE assays in a quantitative high-throughput screening (HTS) format to test a large collection of chemicals for oxidative stress induction ability. The assay descriptions involve cell handling, assay preparation, instrument usage, and assay procedure.
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Key words
Antioxidant response element (ARE),KeratinoSens,Luciferase (luc),Nuclear factor erythroid 2-related factor 2 (Nrf2),Reactive oxygen species (ROS),β-Lactamase (bla)
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