Nuclear lamina strain states revealed by intermolecular force biosensor

We developed an intramolecular FRET-based strain biosensor using nanobodies capable of measuring the mechanical strain of lamin filaments. Using this sensor, we were able to show that the nuclear lamina is subjected to significant strains. These strains are dependent on nuclear volume, actomyosin contractility, functional LINC complex, chromatin condensation state, cell cycle, and EMT. Overall, we demonstrate that nanobody-based approach allows construction of novel force biosensors for mechanobiology studies. ### Competing Interest Statement The authors have declared no competing interest.