Improved Loop Mediated Isothermal Amplification (LAMP) Assay for Visual, Rapid and Sensitive Detection of Canine Parvovirus Antigenic Types

Canine parvoviral enteritis remains one of the dreaded enteric viral diseases of dogs. Rapid detection of the etiological agent, Canine parvovirus-2 (CPV-2) is required for managemental intervention and initiating therapeutic and control measures. The present study describes Loop Mediated Isothermal Amplification (LAMP) using loop primers, validating the assay on all the circulating CPV-2 antigenic types and testing in 120 suspected clinical samples. The novel LAMP assay was completed within 30-45 min at 64°C in a water bath. Analytical sensitivity of the LAMP assay was 100 times more than conventional PCR and was comparable to real-time PCR. LAMP was able to detect 62 positive samples while conventional PCR could detect only in 51 samples. The developed LAMP provides a simple, rapid, cost-effective, sensitive, specific method of detection of CPV-2 infection in dogs in clinical settings.