Simultaneous determination of selected pesticides and/or their metabolites in urine by off-line solid phase extraction and ultra high performance liquid chromatography/hybrid quadrupole time-of-flight mass spectrometry

•Simultaneous determination of selected pesticides and metabolites in human urine.•UHPLC/QTOFMS coupling perfectly adapted to targeted quantitative analysis.•Deconjugation step essential to measure the total concentration of certain substances.•Likely impact of the thawing/freezing cycles on the stability of certain substances.•Presence of pesticides in their metabolized form in French pregnant women's urine.