Rapid UV-Vis spectroscopy methods for quantification of ranitidine tablets

Ranitidine is a histamine H2-receptor antagonist that inhibits gastric acid secretion, commonly used in the treatment and prophylaxis of peptic ulcer and gastroesophageal reflux disease. Over-the-counter ranitidine hydrochloride tablets are available in several European countries as well as the US for the relief of indigestion and heartburn. Commercial tablets containing the equivalent of 150 mg of ranitidine were analysed following extraction. Ranitidine is water-soluble, thus avoiding the use of organic solvents. Standard aqueous solutions of ranitidine hydrochloride were used to construct a calibration curve. This graduate laboratory experiment describes a simple, inexpensive, safe, and “green” procedure for the quantification of ranitidine tablets by UV spectrophotometry. The entire procedure provides an pedagogical opportunity to approach a realistic analytical scenario, typical of a quality control laboratory, by designing an attractive and engaging hands-on session. An average content of 148 ± 1 mg of ranitidine was determined using both standard addition and external calibration methods and results are in agreement with Pharmacopoeia. Keywords: Analytical chemistry, ranitidine, drugs/pharmaceuticals, quantitative analysis, UV-Vis spectroscopy