Comparative assessment of polyherbal formulation and Mucuna pruriens extract as neuroprotectant by using MPTP screening mouse model of Parkinson disease

Background: The main aim of the study was to compare the neuroprotective potential of Polyherbal Formulation (PHF) with respect to an extract of well-reported anti-parkinson plant i.e. Mucuna pruriens. Methods: Different PHF combinations (PHFs) were formulated by using hydro-alcoholic extracts and were tested for neuroprotective potential against Mucuna pruriens extract (MPE). In experimental study, 30 albino mice (Swiss strain, 35-45g) were grouped into Control, MPTP, MPTP+MPE, MPTP+PHFs, MPTP+ L-DOPA. Experimental mice were given PHFs and MPE (50 mg/kg body wt.) by intraperitoneal routes. MPTP (1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine ) treatment was given orally for 2 week with prior use of 20 mg/kg body wt. for 2 weeks. After treatment, a neurobehavioral study as well as neurochemical parameter was performed. Results: The Result mentioned that polyherbal formulation improved better the dopaminic neurons in the substantia nigra region of the brain compared to MPE with respect to MPTP intoxication. A significant diminishing was found in spontaneous locomotor activity and rota rod activity in MPTP treated mice when contrasted with the control group, which was restored by MPTP+MPE and MPTP+PHF1 treated group when contrasted with the standard L-Dopa treatment group. This improvement was observed to be significantly better in MPTP+PHF1 treated group when contrasted with the treatment of MPTP+MPE. The changes in different parameters happened after MPTP treatment. These changes include levels of Malondialdehyde (MDA), Conjugated Dienes (CD), Superoxide Dismutase (SOD), and Catalase. Conclusion: The study concluded that PHF treatment promotes significant neurogenesis and reduces apoptosis, promotes antioxidant capacity, restores dopamine labels. PHF contains numerous classes of chemical constituents which show a synergistic effect for better therapeutic remuneration and neuroprotection compared to the single chemical entity L-DOPA that is a well-known chemical constituent present in MPE.