Assessment of the R2R3 MYB gene expression profile during tomato fruit development using in silico analysis, quantitative and semi-quantitative RT-PCR

•The expression of R2R3 MYB gene shed the light on its potential function.•The three approaches; qRT-PCR, semi-qRT-PCR and TEA analysis showed similar expression patterns.•The in vitro and in silico expression of R2R3 MYB provide molecular insights into fruit development.•Phylogeny analysis suggests a potential expression profiles in different important plants.