Detection of NDM-1/5 and OXA-48 co-producing XDR hyper-virulent Klebsiella pneumoniae in Northern Italy.

Using a hybrid long reads sequencing approach, we aimed to fully characterize four hypervirulent extensively drug resistant (XDR) isolates of Klebsiella pneumoniae, one of which represented the first strain isolated in Italy co-expressing NDM-1/5 and OXA-48 carbapenemases.Whole Genome Sequencing (WGS) was performed using Illumina and Oxford Nanopore Technology platforms. An assembly pipeline was used to recover the structures of both chromosome and plasmids.Multi-locus sequence typing (MLST) showed that these strains belonged to high-risk sequence types (ST) not commonly circulating in Italy (ST 383, ST147, and ST15). The hybrid sequencing approach allowed to characterize three multi-drug resistance plasmids, which demonstrated high homology with previously sequenced ones, that were simultaneously detected in one ST383 strain, carrying respectively blaNDM-1, blaNDM-5, and blaOXA48.This is the first report in Italy of new hyper-virulent XDR Klebsiella pneumoniae clones characterized by co-production of OXA-48, NDM-1 and NDM-5. The discovery of new high-risk clonality housing multiple mobile elements is a growing problem that poses a great challenge for public health.