Human pituitary growth hormone. XIX. The primary structure of the hormone.

Abstract The isolation and partial characterization of human pituitary growth hormone (HGH) were first reported from this laboratory in 1956 (1). In the ensuing years a number of reports (2) have described improved methods of isolation and further characterization of the hormone as a single polypeptide chain molecule containing a single tryptophan residue, four half-cystine residues, and having a molecular weight of 21,500. Additional information about the sequence of amino acid residues comprising the NH2-terminus (3) and the COOH-terminus (4) of the protein has been published. Herein is the completed account of the total amino acid sequence in HGH. A preliminary report on this work has been published (5).