Photoradiation Effects Using the Combination of HpD and Argon Laser Against Rat Urinary Bladder Cancer (R9061)

FANFT induced transplantable rat urinary bladder cancer line (R9061) was used for this experiment. The cell suspension contain ng 2×106 cells/ml was implanted into the bladder wall of a female Fisher rat.In order to investigate photoradiation effect using the combination of Hematoporphyrin derivative (HpD) and Argon LASER, HpD of 2.5mg/kg dose was administered to each rat on the 5th day. Seventy two hours after administration of HpD, the rat exposed bladder received Argon LASER beam irradiation at a distance of 10cm from guartz tip to tumor. We could obsrve red fluorescence on the tumor by Argon irradiation. Results as shown would prove to be photoradiation effect with the combination of HpD and Argon LASER irradiation. M of the nontreated control group was 1.44g (n=4). M of the 60 sec. Argon irradiation without HpD was 0.988g( n=5). M of the 10 sec. Argon irradiation group with HpD was 0.49g (n=4) M of the 60 sec. Argon irradiation group with HpD was 0.62g (n=5). (M: average bladder weight)There is a statistically significant difference between the nontreated control group and both the 10 sec. Argon group and the 60 sec. Argon group with HpD.(p<0.05)