Experimental studies of mouse CD4~+CD25~-T cells transduced with Forkhead Box P3 gene recombinant adeno-associated virus vector

Objective:To investigate the expression of Forkhead Box P3(FoxP3) on mouse T cells,and the effects of CD4~+CD25~-T cells transduced with recombinant adeno-associated virus(rAAV) vector carrying FoxP3 gene.Methods:FoxP3 mRNA levels in mouse T cells were analyzed by quantitative real-time PCR.The rAAV vector carrying mouse FoxP3 gene was constructed.CD4~+CD25~-T cells were infected with rAAV-FoxP3 and the functions were analyzed by lymphocyte proliferation assay.Results:CD4~+CD25~+T cells expressed more abundant FoxP3 mRNA than other T cell populations.The rAAV-FoxP3 was successfully constructed.After CD4~+CD25~-T cells were transduced by rAAV-FoxP3,these cells were hyporesponsive to stimulation via their T cell receptor and suppressed the proliferation of freshly isolated CD4~+CD25~-T cells in co-culture experiments.Conclusion:FoxP3-transduced CD4~+CD25~-T cells by rAAV possessed immunosuppression in vitro and will be possibly applied in immune disorders.