Safety evaluation of the rhpro-urokinase

Objective:To probe whether the purified and inactivated recombinant human prou- rokinase(rhpro-UK)carries the infecting retrovirus.Methods:The SRSV/3T3 cell lines and NIH/3T3 cell lines infected by the culture supernatant of Sp2/0 cells were used as positive controls.The NIH/3T3 cell lines were infected by rhpro-UK solutions 1,0.15 and 0.03 mg·mL~(-1),respectively.The virological features of the rhpro-UK in these cell lines were blinded passage 3 generations and then detected by XC cell fusion assay and transmission electric microscope(TEM)technique.Results:No fusion cells and retrovirus granules were found in the rhpro-UK infected NIH/3T3 cell lines,which were significant differ- ent compared with the ones in the positive control cell lines (P 0.01 ).Conclusion:The rhpro-UK has no endogenous retrovirus.