Identification of Tricholoma matsutake in a Pine Root by ITS Region Amplification and RAPD Analysis with Different Mycorrhiza
Journal of the Korean wood science and technology(2006)
摘要
A simple method for identif ying of ectom ycorrhizal fungi was presented, using the pol ymerase chain reaction (PCR) to amplify the ITS (Interna l transcribed spacer) regions of the nuclear ribo-sormal repeat. The sequences analyzed 6 species, Pisolithus tinctorius , Chroogomphus rutilus , Leucogyrophana pinastri , Suillus granulatus , Lactarius laeticolorus , and Suillus bovinus at hon-greung forest, and analysed 10 species, Craterellus lutescens , Thelephoroid mycorrhizal , Lactariu s - 96 -1)ITS 영역 증폭에 의한 소나무 송이균 뿌리 감염 확인 및 RAPD 에 의한 타 균근과의 비교- 97 -quieticolo r, Tricholoma matsutak e, Lactarius chrysorrheu s, Sarcodon aspratu s, Russula versi -color , Suillus luteus , Tricholoma terreum , and Amanita vaginata at hongcheon forest. Finally, th eamplification by PCR with ITS1-ITS4 primers ofers god results over classical identification for ec -tomycorrhizal fungi species.
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关键词
tricholoma matsutake,pine root
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