Ascertainment of effective RNAi sequences of N-ras in humen hepatocellular carcinoma cells

Objective To ascertain the effective RNAi sequences of N-ras in humen hepatocellular carcinoma cell.Methods Using computer network resouces and Oligo6.0 and Blast Research software can gain the effective RNAi sequences of N-ras coding region.The hairpin DNA was designed for forming siRNA in vivo transcription.Results To screen fourteen effective RNAi sequences successfully and the hairpin DNA.Conclusion The effective RNAi sequences of N-ras has been successfully acquired,which may provide the theory foundation for the further experimental researches.The researches would make the important effect on the RNAi treatment,the function study of N-ras in humen hepatocellular carcinoma cells and the new drug design for hepatocarcinoma.