Analysis adventitious shoot polarization and plantlet regeneration of Poplar--Zhonglinmeihe

Inducing adventitious shoots were produced from different explants of Populus sp. by tissue culture on 1/2MS or MS medium with different hormone arrays which were 6-BA plus NAA or IBA. The results showed that the adventitious shoot inducing rate of leafstalk was higher than leaf and stem, the adventitious shoots polarization of stem cambium was also good. The optimal medium of leafstalk was 1/2MS ＋ 6-BA0.5 mg·L~(-1) ＋ NAA0.05 mg·L~(-1) titious shoot rate was 76% ,shoot number was 4.7, adventitious shoots grew very well,inducing advenper leafstalk. Adventitious shoots could become whole plants by adventitious shoots multiplication,strength and radication.