FRAKSINASI DAN IDENTIFIKASI SENYAWA ANTIKANKER EKSTRAK KULIT BATANG Rhizopora mucronata SERTA UJI TOKSISITASNYA TERHADAP LARVA UDANG (Artemia salina Leach)

Investigation the anticancer potency of  R. mucronata  has not been carried yet. This research were aimed to extract the bioactive compound of  R. mucronata  with various organic solvents, examine their toxicity againts  A. salina  Leach larv. and identify the toxic compounds from  R. mucronata  steam bark. The extraction of  R. mucronata  steam bark were peformed by maseration with n-hexane, chloroform, ethylacetate and methanol, repectively. The extracts were examined their toxicity againsts  A. salina  Leach larv. The highest activity extracts was fractionated performed by coloumn chromatography. The fractions respectively was examined their toxicity againts  A. salina  Leach larv. Identification of toxic compound was carry out using gas chromatography-mass spectrometry (GC-MS). The chloroform extracts of  R. mucronata  had toxic character. Toxicity test to  A. salina  Leach larv. The Result showed that the chloroform fraction 1 (C1) of  R. mucronata  steam bark had LC 50  equal to 301.50 ppm. Phytochemical study showed that the active fractions contained terpenoid. Bioactive compound from  R. mucronata steam bark were dioctyl phthalate and cyclopropyle azulene decahydro-1,1,7-trimethyl-4-methylene.