Influence of RNA interference targeting PI3K p85α expression on invasion and metastasis of colorectal cancer cells

Objective:To investigate the influence of RNA interference targeting PI3K p85α on the invasion and metastasis of colorectal cancer cells.Methods:Four shRNA vectors and one negative control vector were designed and stably transfected into LoVo cells.Western blotting analysis was used to screen for the group which had the highest inhibitory rate;the cells were subjected to wound closure assay and transwell assay to determine the migration of LoVo cells after stable transfection.Results:Western blotting analysis showed that cells transfected with shRNA/324 vector had the strongest inhibition of PI3K p85α protein,with the inhibition rate being 77%,and cells in this group were used for wound closure assay and transwell assay.Wound closure assay showed that the healing ability of PI3K p85α-depleted cells was significantly lower than that of the control cells(P0.05).Transwell assay showed that the cells passing the polycarbonate membranes was significantly less in PI3K p85α-knockdown cells than in the control cells(P0.05).Conclusion:Depletion of PI3K p85α protein can obviously inhibit the migration of LoVo cells.PI3K p85α may be a new therapeutic target for treatment of colorectal cancer metastasis.