Study on Detection of Food-borne Pathogenic Bacterias by the Multiplex PCR

Objective To develope a method for detection of Salmonella typhimurium,Bacillus dysenteriae and Listeria monocytogenes by multiplex PCR.Methods Characteristic genes such as VipR,ipaH and hlyA were chosen as the target genes for duplex PCR system.Many factors of duplex PCR were optimized.Results The specificity was satisfactory and the sensitivity was up to 104 copies of plasmids in the system.The whole test can be completed in 3h～5h.Conclusion The method has the superiority in theory and practical application,and also examine three three pathogens once.It can be used in clinical diagnosis.