Generation of human dendritic cells from KG-1 cells in vitro by two-step process

Objective:To explore the technigue ofgeneration of dendritic cells from human leukemia cell line KG-1 after induction in vitro.Methods:Two-step inductions were carried out:At first,KG-1 cells were treated with Flt3-ligand,TPO and SCF for 5 days.Then the cells were exposed to factors including GM-CSF,IL-4 and TNF-αfor 7 days to acquire the properties of mature DC.In day 7,the cells morphology were observed under invert microscopy and the phenotype markets(CD40,CD80,CD86,HLA-DR)were detected with FACS.Results:After induction with cytokines,KG-1 exhibited morphologic and phenotypic properties of typical dendritic cells.Conclusion:It is possible to generate typical morphological and phenotypic DCs from KG-1 cells by two step process differentiation.