In vitro studies of the effect of theophylline and/or N-acetylcysteine on redox homeostasis and ketogenesis in rat hepatocytes
Bulletin of The Veterinary Institute in Pulawy(2011)
Abstract
The examinations were conducted on hepatocytes isolated by means of enzymatic method from the liver of three-month-old Wistar rats. The cells were incubated in medium with addition of theophylline and/or N-acetylcysteine. Significant changes in the activity of SOD, GPx, and GR in hepatocytes incubated in the presence of the compounds in comparison with control cells demonstrated that theophylline and/or N-acetylcysteine disturb oxidative-reductive homeostasis of the cells. Changes in concentrations of ketone bodies, resulting in disturbances of acetoacetate to β-hydroxybutyrate molar ratio, point to an unfavourable interference of theophylline into ketogenesis, which is equivalent with the disturbance of the balance between NAD + and NADH+H + in hepatocytes. N-acetylcysteine simultaneously present with theophylline in incubation medium exerted a protective action on ketogenesis.
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Key words
redox homeostasis,theophylline,ketogenesis,n-acetylcysteine
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