Determination of Cyromazine and Dicyclanil Residues on Greasy Wool by High Performance Liquid Chromatography

A method for the determination of cyromazine and dicyclanil residues on greasy wool was developed with HPLC and confirmed with HPLC-MS/MS.The cyromazine and dicyclanil residues on greasy wool were extracted with 1% trichloroacetic acid solution with ultrasonic,and cleaned up by MCX SPE column.The HPLC separation was performed on a Hypersil NH2 using water-acetonitrile(V/V) as the mobile phase with gradient elution and detected at the wavelength of 214 nm.The corroboration method of HPLC-MS/MS was used with electro-spray ionization of positive ion mode.The [M + H]+ and characteristic ions of dicyclanil were m/z 191.0,150.0 and 163.0,and cyromazine were m/z 167.0,85.0 and 125.0.The linear ranges of cyromazine and dicyclanil were 0.05-5.0 mg/L.There were good linear relationships between the peak area and concentration in the linear range.The correlation coefficient was 0.9999.The detection limit of cyromazine was 0.02 mg/kg,and dicyclanil was 0.01 mg/kg.The average recoveries of cyromazine and the dicyclanil were 95.0%-99.9% and 83.6%-92.2%,respectively.