The Influence of Pine Nut Shell Polysaccharide on Immunological Function of Mouse

Objective To study the influence of pine nut shell polysaccharide (PSP) on mouse splenic lymphocytes. Methods Proliferation of T and B lymphocytes induced by ConA or LPS were determined by using MTT assay. Peritoneal macrophage function was determined by using neutral red approach. NK cell competence was determined by using lactate dehydrogenase approach. Results The results showed that PSP cell toxicity to splenic lymphocytes is very low,it remained higher immunologic competence on splenic lymphocytes even if the polysaccharide density achieves 1000 μg/mL. PSP of different concentrations can promote T and B Lymphocyte proliferation,and PSP significantly enhanced T lymphocyte proliferation induced by ConA ranging from 100 μg/mL to 300 μg/mL (P 0. 01). Different density PSP could enhance the function of peritoneal macrophage englobing neutral red and its metabolism. PSP could improve effect of NK cells against YAC-1. Conclusions PSP has the immunity activeness strongly,the toxic low characteristic. Thus,this polysaccharide seems to be a good candidate for the development of immunity conditioner.