Abstract 2288: CYP3A4 monoxygenase regulates IGF-1 signaling in ER-positive breast cancer cells

Cytochrome P450 3A4 (CYP3A4) is an important monooxygenase that metabolizes a wide range of xenobiotics including many drugs and environmental chemicals, as well as endogenous substances such as steroids. Expression of CYP3A4 has been observed in breast tumors and has been associated with nodal metastases and decreased overall survival in breast cancer. To investigate roles of CYP3A4 on breast cancer progression and the mechanisms involved, we performed RNAi knock down studies, demonstrating that CYP3A4 regulates anchorage-dependent proliferation and clonogenicity of the MCF7 line. Unexpectedly, we found that stable CYP3A4 shRNA knock down MCF7 lines exhibited significant higher clonogenicity relative to the non-target control line in soft agar (P Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2288. doi:10.1158/1538-7445.AM2011-2288