An improved method for primary culture of mouse pulmonary microvascular endothelial cells

Objective To establish an improved method for culturing primary mouse pulmonary microvascular endothelial cells (PMVECs). Methods An improved tissue block adherent culture method was used to isolate and culture the PMVECs from C57 mice. The cultured cells were identified by factor VIII-related antigen and CD31 antigen, and the growth of cells cultured using the improved method and the conventional method was compared. Results The cultured primary pulmonary microvascular endothelial cells showed a short fusiform or round morphology, and the cell monolayer displayed a cobble stone-like appearance. The cultured cells were positive for VIII-related antigen and CD31 antigen. The cell growth was accelerated in the cell cultures with the improved method compared with that in conventional cell cultures. Conclusion The improved culture method allows more efficient acquisition of primary mouse PMVECs of a greater purity.