Optimization of Condition for The Fermentation Process of Streptococcus Pneumoniae Serotype 5

Objective:To investigate a good fermentation process of Streptococcus pneumoniae serotype 5 and capsular polysaccharide purification process of Streptococcus pneumoniae serotype 5.Methods: Streptococcus pneumoniae serotype 5 was cultured in 70 L fermenter with orthogonal experimental design,and purifying capsular polysaccharide.Results: The main quality indexes of the capsular polysaccharide of Streptococcus pneumoniae serotype 5 met the requirements of Europe Pharmacopeia 5.0.Conclusion: A successful process for large-scale production of Streptococcus pneumoniae serotype 5 capsular polysaccharide was established,and this procedure is suitable for pneumococcal vaccine production.