Construction of BD Fusion Vector pGBKT7-Ts with T_(1083) Substitution in of NtKRP Tail
Journal of Anhui Agricultural Sciences(2010)
Abstract
[Objective]The aim was to construct BD fusion vector pGBKT7-Ts with T1083 substitution in NtKRP tail. [Method]T1083 substitution mutant in tail sequence of tobacco NtKRP was amplified by overlapping PCR from pBI121KE plasmid template,and the fragment was cloned into pGBKT7 vector. [Result]BD fusion expression plasmid pGBKT7-Ts was constructed successfully. [Conclusion]The study laid an experimental foundation for further understanding of the effect of T1083 substitution mutation on the interaction of NtKRP and its target partner.
MoreTranslated text
Key words
fusion
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined