Construction of BD Fusion Vector pGBKT7-Ts with T_(1083) Substitution in of NtKRP Tail

[Objective]The aim was to construct BD fusion vector pGBKT7-Ts with T1083 substitution in NtKRP tail. [Method]T1083 substitution mutant in tail sequence of tobacco NtKRP was amplified by overlapping PCR from pBI121KE plasmid template,and the fragment was cloned into pGBKT7 vector. [Result]BD fusion expression plasmid pGBKT7-Ts was constructed successfully. [Conclusion]The study laid an experimental foundation for further understanding of the effect of T1083 substitution mutation on the interaction of NtKRP and its target partner.