Content Determination of Chlorogenic Acid in Mori Cortex by HPLC

Objective:To establish a HPLC method for the content determination of chlorogenic acid in Mori Cortex.Method: The central composite rotatable design was used to optimize extracted conditions.The content of chlorogenic acid in Mori Cortex was determined by HPLC on a Diamonsil C18 column(4.6 mm×250 mm,5 μm)detected at the wavelength of 327 nm with acetonitrile-0.4% phosphoric acid(11∶89)solution as the mobile phase,the flow rate was 1 mL·min-1.Result: The optimum extractable conditions to obtain desirable extraction yields for chlorogenic acid were found at the methanol concentration of 60%,extraction time of 45 min and solvent quantity of 10 time,chlorogenic acid was linear in the range of 0.045-0.45 μg,regress equation was Y=2 329.028 9X+8.184 1(r=0.999 9),the average recovery was 97.89% with RSD of 3.14%.Conclusion: The method of HPLC determination is simple,reliable and accurate and can be used for the quality control of Mori Cortex.