Expression of IL-23 receptor on spleen CD4~+T lymphocyte in mouse and its significance in bronchial asthma

Objective To detect the expression of IL-23 receptor on spleen CD4+T lymphocytes in mouse model of acute bronchial asthma (asthma)as well as its significance in the pathogenesis.Methods Spleen CD4+T lymphocytes origin from spleen of mouse model of acute asthma isolated by immunomagnetic beads and they were used to determine the expression of IL-23 receptor mRNA by real-time PCR,the level of IL-23 receptor protein by Western blotting,the ratio of positive Th17 cells by flow cytometer and the production of IL-17 by ELISA.Results The expression of IL-23 receptor,the ratio of Th17 cells and the production of IL-17 on Spleen CD4+T lymphocytes in asthmatic model was obviously higher than that of the control group.The expression of IL-23 receptor on spleen CD4+T lymphocytes positively correlated with the ratio of positive Th17 cells and the production of IL-17.Conclusions IL-23 receptor possibly plays an important role in the pathogenesis of asthma.