Effects of valsartan on activity of matrix metalloproteinase-2 in rat vascular smooth muscle cells induced by angiotensin II
Medical Journal of the Chinese People's Armed Police Forces(2010)
Abstract
Objective To study the effect of valsartan on the activity of MMP-2 in isolated rat vascular smooth muscle cells (VSMCs)stimulated by angiotensin Ⅱ(Ang Ⅱ).Methods VSMCs from the rat thoracic aorta were cultured by attachment-block culture.VSMCs were treated with angiotensin Ⅱ and valsartan.The activity of matrix metalloproteinase-2(MMP-2)was determined by zymogram.Results Angiotensin Ⅱ significantly enhanced the activity of MMP-2 of VSMCs.Observation was made at four time points:6 h,12 h,24 h and 48 h.The activity of MMP-2 was strongest at 12 h,and then gradually decreased.Therefore,12 h was selected as a time end point to observe the drug effect,and valsartan significantly inhibited the effect of angiotensin Ⅱ on the activity of MMP-2 with significant statistical difference.Conclusions Angiotensin Ⅱ enhances the activity of MMP-2 of VSMCs.Valsartan inhibits the activity enhancement of MMP-2 of VSMCs induced by angiotensin Ⅱ.
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Key words
Matrix Metalloproteinases
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