Determination of benzopyrene in food by solid phase extraction-high performance liquid chromatography with fluorescent detector

Objective A method of solid phase extraction-high performance liquid chromatography with fluorescent detector(HPLC-FLD)was developed for determination of benzopyrene(BaP) in foodstuffs.Method The analytical procedure includes optimization of extraction conditions,purification using a SPE column and separation by XbridgeTMC18Chromatographic column,followed by a quantitative external standard method.Results The method showed a good linearity over the range of 0~100 ng/mL for benzopyrene(BaP) with r=0.9994.The detection limits(S/N≥10) were 1.0 μg/kg for tea seed oil,tilapia,pork and 0.5 μg/kg for rice,cabbage,tea,water and orange juice.The recoveries were 77.3%~93.2% at spiked levels of 0.5 μg/kg~100 μg/kg and the relative standard deviations were 0.65%~7.18%(n≥6).Conclusion This method has the advantages of easy to operate,fast to perform,with high sensitivity and accuracy,and it was suitable for the detection of benzopyrene in foodstuffs.