Study of anti-melanoma effect in mice injected with melanoma cells transfected with the recombinant expressing Ag85A and GM-CSF

Objective:To study anti-melanoma effect in mice injected with melanoma cells expressing Mycobacterium tuberculosis antigen 85A(Ag85A) and GM-CSF as heterogeneity antigen and immunoadjuvants.Methods:The recombinant pRSC-Ag85A/GM-CSF was constructed by the molecular biological technology and was transfected into the melanoma B16 cells and the transcription of Ag85A and GM-CSF in B16 cells was respectively detected by RT-PCR.The different gene-modified B16 cells were injected into C57BL/6 mice and the tumorigenicity was analyzed by measuring the size of tumor and observing the survival of tumor-bearing mice.Meanwhile,the anti-tumor immune response induced by the Ag85A and GM-CSF was evaluated by detecting the activities of CTL and NK cells,and serum cytokine level respectively.Results:Mice injected with B16 cells transfected with the recombinant pRSC-Ag85A/GM-CSF could survive longer than the other mice.Compared with the control group,the level of IFN-γ in the serum and the activities of CTL and NK cells were obviously increased and the difference was statistically significant.Conclusion:B16 cells transfected with the recombinant pRSC-Ag85A/GM-CSF could reduce the tumorigenicity and effectively induce the anti-tumor effect in mouse model.