Influence of zinc phosphate mineralized collagen membranes on behavior of MG63 cells in vitro

Objective:This study was to determine the effect of zinc phosphate mineralized collagen membranes on the proliferation and differentiation of human osteoblast-like cells MG63 in vitro.Method:The collagen membranes were mixed with zinc phosphate by the method of immersing into the mineralization solution.Cells from an established osteoblast-like line(MG63)were cultured on membranes.Cell morphology attached to the membranes was evaluated by scanning electron microscope.The quantity and ALP activity of cells were determined by CCK-8 assay and ALP activity test.Result:MG63 cells attached to the membranes and extended pseudopod.Compared to the control,zinc phosphate mineralized collagen membranes significantly increased the proliferation of MG63 on the 3rd day and no significant influence on the other cultured days was observed.The ALP activity of MG63 planted on zinc phosphate mineralized collagen membranes was significantly higher than the control on the 1st day,while no significant influence on the other cultured days was observed.Conclusion: Zinc phosphate mineralized collagen membranes possess excellent biocompatibility.