P0110 : Activation of myostatin and MuRF-1 is involved in muscle wasting in mice model of primary biliary cirrhosis

severe liver cirrhosis and in patients with mild or no fibrosis along with biopsies of normal livers. Results: CTSL was found to be significantly up regulated in CCL4 induced fibrotic mice (n =5) at both RNA and protein level as compared to control mice olive oil treated (n =6) while no significant change was evident in CTSB levels. The level of CTSL was found to be significantly upregulated by 1.5 and 1.8 fold in mdr2 as compared to wild type controls in 4 (n =7) and 8 weeks (n =8) old group of mice respectively whereas no changes was observed in 16 week old mdr2 mice as compared to the age matched wild type controls. Similarly CTSB expression was observed to be significantly higher in 4 week old mdr2 mice as compared to the wild type counterparts where as 8 and 16 old cohort of mice did not show any significant changes. Similar results were obtained when liver tissues from these groups of mice were stained for CTSL and CTSB. CTSL was found to be upregulated in liver samples of fibrotic patients (n = 10) as assessed by IHC and its expression increases with increasing grade of liver fibrosis. However, no significant changes in CTSB expression were observed in these samples. Conclusions: CTSL expression increases with the extent of the severity of liver fibrosis in different murine models as well as in patient diagnosed with this pathology.