Influence of high-fat diet on aquaporin 7 in perirenal adipose of male DIO rats

Objective: To observe the expression of aquaporin 7(AQP7) mRNA in perirenal adipose of dietary induced obese rats and explore the role of AQP7 in obesity development. Methods: 48 male Sprague-Dawley rats were randomly divided into the control group(C group) and the model group(DIO group), which were fed with normal and high-fat diet, respectively. Rats in DIO group were screened out according to body weights after 8 weeks and fed with high-fat diet for another 12 weeks. The rats of both groups were sacrificed after 8 and 20 weeks. Lee's index, liver/body weight, fat content and serum lipid were measured. AQP7 mRNA expression in perirenal adipose was determined by Real-time PCR.Results: At 8th week, body weight, Lee's index, liver/body weight, fat content and serum TG levels in DIO group were higher than in C group(P0.01 or P0.05), while these indicators were further increased(P0.01) at 20th week and serum TC and LDL-C levels were also higher than in C group(P0.01 or P0.05). AQP7 mRNA expression in perirenal adipose of DIO group were up-regulated(P0.01) at 8th week and down-regulated(P0.05) at 20th week. Conclusions: AQP7 expression in perirenal adipose might play different roles in obesity of different degrees.