Using cost-effective 454 GS-FLX Titanium sequencing technology,
microsatellite markers were isolated de novo for Barbus
meridionalis. Five multiplex sets including 28 newly and two
previously-developed loci were optimized using allopatric
populations of both B. barbus and B. meridionalis. Our study
revealed the high success rate of cross-species amplification
in two Balkan species, B. cyclolepis and B. balcanicus.