Innovative no-wash immunoassay combining magnetic nanoparticles and micro-magnets

We present an innovative, one-step, no-wash fast immunoassay exploiting functionalized nanoparticles, structured micro-magnets and fluorescent detection: MagIA. As a proof of concept, we compare MagIA and standard ELISA performances applied to ovalbumin monoclonal antibody detection (anti-OVA mAb) in buffer. We then achieve the detection of anti-OVA mAb in biological fluid (mouse serum 1:100). Finally, MagIA protocol is transferred to clinical parameters measurements: CRP and Toxoplasma gondii serology. Comparison of data obtained with automated immuno-analysers shows excellent correlation.