Regenerasi Pepaya melalui Kultur In Vitro

A study was conducted in the Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development to optimize papaya regeneration systems through in vitro culture. Four steps were done, i.e., callus induction, callus regeneration, root formation, and acclimatization. Explant materials used were immature embryos of papaya cv. Burung. Immature papaya embryos were cultured on different media. The best medium for embryogenic callus development was ½ MS + 10 mg/l 2.4-D + 60% sucrose + 143 mg/l adenine sulphate + 50 mg/l myo inositol + 400 mg/l glutamine, while that for callus embryo regeneration was MS + 0.5 mg/l GA3 + 0.1 mg/l kinetin + Morel and Wetmore Vitamin. Using this medium, the average of shoot formation was three shoots per explant of embriogenic callus, and the percentage of regenerated callus was 80%. The color of shoot derived from this treatment was green. Eighty percent of plants formed a complete root development using ½ MS + 0.5 mg/l paclobutrazol media. Media hull of rice and compost was the best medium for papaya plant acclimatization. The percentage of survival on that acclimatization step was 65%.