In vitro propagation of Kaempferia galanga zingiberaceae and comparison of larvicidal activity and phytochemical identities of rhizomes of tissue cultured and naturally grown plants

Vegetative propagation of K galanga Linn Zingiberaceae cannot fulfill the current demand for planting material and tissue culture offers an alternative means for mass propagation Experiments were carried out on propagation of plants through direct organogenesis and comparison of larvicidal activity and phytochemicals present in rhizome of natural and tissue cultured plants in order to confirm the potential use of tissue cultured plants as an alternative to natural plants in commercial scale productions In vitro shoot induction was optimized with rhizome bud explants grown on MS medium supplemented with mg L Benzyl amino purine BAP and mg L IAA Indole acetic acid MS medium supplement with mg L IAA and mg L Indol butric acid IBA was identified as the best medium for root induction Rooted plantlets were acclimatized successfully in a mixture of soil sand compost Hexane found to be a better solvent for extraction of phytochemicals over methanol and the hexane extract showed the highest larvicidal activity against the fourth instar larvae of A aegypti GC MS analysis revealed the existence of the nine key compounds in both samples tested confirming the possibility of using tissue cultured plants as a substitute for natural plants in medicinal purposes