Анализ результатов применения различных антител для определения экспрессии CD1a при иммунофенотипировании т-линейного острого лимфобластного лейкоза у детей

CD1a antigen expression is an important prognostic factor in T-lineage acute lymphoblastic leukemia (T-ALL), thus standardized approach for this antigen expression detection is crucial for multicenter trials. The use of different antibodies in laboratories could lead to wrong decisions for patients management. The aim of the present study was to analyze the results of flow cytometric bone marrow investigation in children with T-ALL using different CD1a-directed antibodies. The bone marrow samples from 31 children (8 girls and 23 boys) with T-ALL aged from 1 to 16 years (median age 7) were studied by multicolor flow cytometry including two different antibodies against CD1a (BL6 и SK9). There were no significant differences in the immunophenotyping results. However, the CD1a-positivity of tumor cell population was visible in the dot plot better when BL6 antibody was used. In addition, two patients with discordant CD1a expression data were founded. Therefore, we antibody BL6 was recommended for routine T-ALL immunophenotyping.